Variabilities and similarities of adult stem cells derived intestinal organoids originating from different intestinal segments in pig

Author:

Kar Soumya K.,te Pas Marinus F.W.,Rikkers Roxann,Madsen Ole,Taverne Nico,Ellen Esther D.,Wells Jerry M.,Schokker Dirkjan

Abstract

1.ABSTRACTOrganoids arein vitromodel systems generated from tissues. Organoids express specific physiological functions associated with their original tissue location and they express tissue-segment-specific genes. The aim of this study was to culture pig organoids from different areas of intestinal segments: duodenum, ileum (with or without Peyers Patches (PP)), and colon, to investigate the resemblance with thein vivotissues and variability of multiple adjacent sampling sites based on histology and transcriptome profiles. The transcriptome profiles of thein vivotissues and the derived organoids showed high resemblance for all intestinal segments. For the transcriptomic cluster analysis it was shown that it is important to use tissue important genes to shown the resemble between tissue and their derived organoids. The transcriptome profiles clearly separated the intestinal segments, and samples of the same segment from adjacent tissue locations showed high transcriptome profile similarity. Ileum samples with and without PP were also separated. Pathway analysis of differentially expressed genes from PP compared with non-PP suggested the importance of several aspects of cell cycle progression regulation, including DNA metabolism, chromatin organization, regulation of mitotic stage progression, and regulation of inflammation. Based primarily on the transcriptomics results, we conclude that organoids reflect the sampled intestinal segment and that organoids derived from adjacent sampling sites in an intestinal tissue segment showed low variability. The results from the ileum indicate that organoids have potential to study intestinal innate immune processes.

Publisher

Cold Spring Harbor Laboratory

Reference18 articles.

1. Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform

2. Live imaging of cell division in 3D stem-cell organoid cultures

3. Development of intestinal organoids as tissue surrogates: Cell composition and the Epigenetic control of differentiation

4. Germ-free and microbiota-associated mice yield small intestinal epithelial organoids with equivalent and robust transcriptome/proteome expression phenotypes;Cell. Microbiol.,2020

5. Organoids: a promising new in vitro platform in livestock and veterinary research;Vet. Res.,2021

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3