Abstract
AbstractPurposeTo isolate the structural components of theex vivoporcine iris tissue and to determine their biomechanical properties.MethodsThe porcine stroma and dilator tissues were separated, and their dimensions were assessed using optical coherence tomography (OCT). The stroma underwent flow test (n= 32) to evaluate for permeability using Darcy’s Law (ΔP= 2000 Pa,A= 0.0391 mm2), and both tissues underwent stress relaxation experiments (ε= 0.5 with initial ramp ofδε= 0.1) to evaluate for their viscoelastic behaviours (n= 28). Viscoelasticity was characterised by the parametersβ(half width of the Gaussian distribution),τm(mean relaxation time constant),E0(instantaneous modulus) andE∞(equilibrium modulus).ResultsFor the stroma, the hydraulic permeability was 9.49 ± 3.05 × 10-5mm2/Pa·s, and the viscoelastic parameters wereβ= 2.50 ± 1.40, andτm= 7.43 ± 4.96 s, with the two moduli calculated to beE0= 14.14 ± 6.44 kPa andE∞= 6.08 ± 2.74 kPa. For the dilator tissue, the viscoelastic parameters wereβ= 2.06 ± 1.33 andτm= 1.28 ± 1.27 s, with the two moduli calculated to beE0= 9.16 ± 3.03 kPa andE∞= 5.54 ± 1.98 kPa.ConclusionWe have established a new protocol to evaluate the biomechanical properties of the structural layers of the iris. Overall, the stroma was permeable and exhibited smaller moduli than those of the dilator muscle. An improved characterization of iris biomechanics may form the basis to further our understanding of angle closure glaucoma.
Publisher
Cold Spring Harbor Laboratory