Signal detectability and boldness are not the same: the function of defensive colouration in nudibranchs is distance-dependent

Abstract

AbstractAposematic signals advertise underlying defences in many species. They should be detectable (highly contrasting against the background) and bold (high internal pattern contrast) to enhance predator recognition, learning and memorisation. However, the signalling function of aposematic colour patterns may be distance-dependent: signals may be undetectable from a distance to reduce costs of increased attacks from naïve predators but bold when viewed up close. To test this hypothesis, we quantified the chromatic and achromatic detectability and boldness of colour patterns in 13 nudibranch species that varied in the strength of their chemical defences, in terms of unpalatability and toxicity, using Quantitative Colour Pattern Analysis (QCPA) and data on the visual perception of a triggerfish (Rhinecanthus aculeatus). When viewed from larger distances, there were no differences in detectability and boldness between well-defended and undefended species. However, when viewed at close distances, well-defended species were more detectable and bolder than undefended species. The detectability of defended species decreased more significantly with increased viewing distances compared to boldness but remained relatively consistent over viewing distances for undefended species. We provide evidence for distance-dependent signalling in aposematic nudibranchs and highlight the importance of distinguishing between signal detectability and boldness in studies of aposematism.