Transcriptional profiling of rare acantholytic disorders suggests common mechanisms of pathogenesis

Abstract

AbstractBackgroundDarier, Hailey-Hailey, and Grover’s diseases are rare non-autoimmune acantholytic skin diseases. While these diseases have different underlying causes, they share defects in cell-cell adhesion in the epidermis and desmosome organization.ObjectiveTo better understand the underlying mechanisms leading to disease in these conditions we performed RNA-seq on lesional skin samples from Darier, Hailey-Hailey, and Grover’s disease patients.MethodsRNA-seq and bioinformatics analyses were performed on banked paraffin embedded diagnostic samples from each disease. For detailed Methods, please see the Methods section in this article’s Online Repository atwww.jacionline.org.ResultsThe transcriptomic profiles of Darier, Hailey-Hailey, and Grover’s disease were found to share a remarkable overlap, which did not extend to other common inflammatory skin diseases, psoriasis and atopic dermatitis. Analysis of enriched pathways showed a shared upregulation in keratinocyte differentiation and Th17 inflammatory pathways, and a decrease in cell adhesion and actin organization pathways in Darier, Hailey-Hailey, and Grover’s disease. Direct comparison to atopic dermatitis and psoriasis showed that the downregulation in actin organization pathways was a unique feature in Darier, Hailey-Hailey, and Grover’s disease.Further, upstream regulator analysis suggests that a decrease in SRF/MRTF activity may be responsible for the downregulation of actin organization pathways. Staining for MRTFA in lesional skin samples showed a decrease in nuclear MRTFA in patient skin compared to normal skin.ConclusionThese findings highlight the significant level of similarity in the transcriptome of Darier, Hailey-Hailey, and Grover’s disease, and identify decreases in actin organization pathways as a unique signature present in these conditions.Key MessagesDarier Disease, Hailey-Hailey Disease, and Grover’s Disease share similar transcriptional profiles suggesting common mechanisms of pathogenesis.SRF/MRTFA activity is reduced in Darier Disease, Hailey-Hailey Disease and Grover’s disease, implicating actin organization in acantholysis.