Abstract
ABSTRACTSummaryBiological condensates are membraneless organelles with different material properties. Proteins and RNAs are the main components, but most of their interactions are still unknown. Here we introduce PRALINE, a database for the interrogation of proteins and RNAs contained in stress-granules, processing bodies, and other assemblies including droplets and amyloids. PRALINE provides information about the predicted and experimentally validated protein-protein, protein-RNA and RNA-RNA interactions. For proteins, it reports the liquid-liquid phase separation and liquid-solid phase separation propensities. For RNAs, it provides information on predicted secondary structure content. PRALINE shows detailed information on human single-nucleotide variants, their clinical significance and presence in protein and RNA binding sites, and how they can affect condensates’ physical properties.AvailabilityPRALINE is freely accessible on the web athttp://alvinlee.bio.uniroma1.it/praline.Supplementary informationGeneral information is athttp://alvinlee.bio.uniroma1.it/praline/about, where we provide a detailed description of the datasets and the tools employed in the database. Data provided in PRALINE are available athttp://alvinlee.bio.uniroma1.it/praline/downloads. The tutorial is athttp://alvinlee.bio.uniroma1.it/praline/tutorial.
Publisher
Cold Spring Harbor Laboratory