Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets-Reference-Cited by-同舟云学术

Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets

Published:2022-12-01 Issue: Volume: Page:
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Author:

Han Yi,Yang Junqi,Li Yuan,Chen Yu,Ren Huixia,Ding Ran,Qian Weiran,Ren Keyuan,Xie Beichen,Deng Mengying,Xiao Yinghan,Chu Jun,Zou Peng

Abstract

AbstractAs fast developing tools for observing cellular membrane potential, red-emitting genetically encoded voltage indicators (GEVIs) reduce auto-fluorescence background, allow multiplexed recordings, and enable all-optical electrophysiology, but have been limited by either insensitivity or dimness. Here, we report a pair of red GEVIs, Cepheid1b/s, with improved sensitivity, brightness, and photostability. Cepheid1 indicators faithfully report cellular excitability in pancreatic islets and neural activity in acute brain slices.

Publisher

Cold Spring Harbor Laboratory

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