The Arabidopsis LHT1 amino acid transporter contributes toPseudomonas simiae-mediated plant-growth promotion by modulating bacterial metabolism in the rhizosphere

Author:

Agorsor Israel D. K.,Kagel Brian T.,Danna Cristian H.

Abstract

ABSTRACTThe root microbiome structure ensures optimal plant-host health and fitness, and it is, at least in part, defined by the plant genotype. It is well-documented that root-secreted amino acids promote microbial chemotaxis and growth in the rhizosphere. However, whether the plant-mediated re-uptake of amino acids contributes to maintaining optimal levels of amino acids in the root exudates, and in turn, microbial growth and metabolism, remains to be established. Here we show that LHT1, an amino acid inward transporter expressed inArabidopsis thalianaroots, limits the growth of the plant-growth-promoting bacteriaPseudomonas simiaeWCS417r (PsWCS417r). Amino acid profiling of thelht1mutant root exudates showed increased levels of glutamine, among other amino acids. Interestingly,lht1exudates or Gln-supplemented wild-type exudates enhancePsWCS417r growth. However, despite promoting bacterial growth and robust root colonization,lht1exudates and Gln-supplemented wild-type exudates inhibited plant growth in aPsWCS417r-dependent manner. Transcriptional analysis of defense and growth marker genes revealed that plant growth inhibition was not linked to the elicitation of plant defense, but likely to the impact ofPsWCS417r amino acids metabolism on auxin signaling. These data suggest that an excess of amino acids in the rhizosphere impactsPsWCS417r metabolism which in turn inhibits plant growth. Together, these results unveil that LHT1 regulates the amino acid-mediated interaction between plants andPsWCS417r and suggest a complex relationship between root-exuded amino acids, root colonization by beneficial bacteria, bacterial metabolism, and plant growth promotion.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3