Author:
Behera Smrutisanjita,Krebs Melanie,Loro Giovanna,Schumacher Karin,Costa Alex,Kudla Jörg
Abstract
Temporally and spatially defined changes in cellular calcium (Ca2+) concentration represent stimulus-specific signals and regulate a myriad of biological processes. The development of ratiometric Ca2+ reporter proteins like Yellow Cameleons (YCs) has greatly advanced our ability to analyze Ca2+ dynamics in vivo with unprecedented spatial and temporal resolution. In plants, the application of these Ca2+ reporter proteins has been pioneered for the investigation of Ca2+ dynamics in guard cells, and recently their use has been extended to other single-cell models like growing pollen tubes and root hairs. However, in plants, the use of YC reporter proteins has largely remained restricted to the investigation of cytoplasmic alterations of Ca2+ concentrations. Here, we provide an introduction to current methods for imaging Ca2+ dynamics with increasing sophistication.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
19 articles.
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