HMGN5, an RNA or Nucleosome binding protein - potentially switching between the substrates to regulate gene expression

Abstract

AbstractThe packaging of DNA into chromatin and its compaction within cells renders the underlying DNA template un-accessible for processes like transcription, replication and repair. Active mechanisms as chromatin modifying activities or the association with non-coding RNAs can de-condense chromatin, rendering it accessible for the DNA dependent processes. High mobility group proteins (HMG) are small architectural chromatin proteins that were shown to contribute to the regulation of chromatin accessibility and condensation. Here we show that HMGN5, a member of the HMGN family that is capable to de-compact chromatin exhibits a novel RNA binding domain that overlaps with its nucleosome binding domain (NBD). HMGN5 binds exclusively to nucleosomes or RNA, suggesting that molecular function relies on switching between these two substrates. We show the specific binding of HMGN5 to regulatory regions and at the same time to bind the RNA of the genes it tends to activate. Furthermore, HMGN5 co-localizes and directly interacts with CTCF, suggesting a cooperative role of both proteins in organizing higher order structures of chromatin and active chromatin domains.