Abstract
AbstractRapid proliferation of the malaria-causing parasitePlasmodium falciparumin the human blood depends on a particularly divergent and acentriolar centrosome, which incorporates several essential centrins. Centrins are small calcium-binding proteins that have a variety of roles and are universally associated with eukaryotic centrosomes. Their precise mode of action, however, remains unclear. In this study calcium-inducible liquid-liquid phase separation is revealed as an evolutionary conserved principle of assembly forPlasmodiumand human centrins. Furthermore, the disordered N-terminus and calcium-binding motifs are defined as essential features for reversible biomolecular condensation and demonstrate that certain centrins can co-condensate. In vivo analysis using live-cell STED microscopy shows liquid-like dynamics of centrosomal centrin. Additionally, implementation of an inducible protein overexpression system reveals concentration-dependent formation of extra-centrosomal centrin assemblies with condensate-like properties. The timing of foci formation and dissolution suggests that centrin assembly is regulated. This study thereby provides a new model for centrin accumulation at eukaryotic centrosomes.
Publisher
Cold Spring Harbor Laboratory
Reference52 articles.
1. WHO. WHO Global, World Malaria Report 2021. Word Malaria report Geneva: World Health Organization. (2021). Licence: CC (2021).
2. Cell division in apicomplexan parasites
3. Apicomplexa Cell Cycles: Something Old, Borrowed, Lost, and New
4. Matthews, H. , Duffy, C. W. & Merrick, C. J . Checks and balances? DNA replication and the cell cycle in Plasmodium. Parasites and Vectors 11, 216 (2018).
5. Gubbels, M. J. , Coppens, I. , Zarringhalam, K. , Duraisingh, M. T. & Engelberg, K . The Modular Circuitry of Apicomplexan Cell Division Plasticity. Front. Cell. Infect. Microbiol. 11, 670049 (2021).
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