The Evolution of Antibiotic Production Rate in a Spatial Model of Bacterial Competition

Abstract

AbstractWe consider competition between antibiotic producing bacteria, non-producers (or cheaters), and sensitive cells in a two-dimensional lattice model. Previous work has shown that these three cell types can survive in spatial models due to the presence of spatial patterns, whereas coexistence is not possible in a well-mixed system. We extend this to consider the evolution of the antibiotic production rate, assuming that the cost of antibiotic production leads to a reduction in growth rate of the producers. We find that coexistence occurs for an intermediate range of antibiotic production rate. If production rate is too high or too low, only sensitive cells survive. When evolution of production rate is allowed, a mixture of cell types arises in which there is a dominant producer strain that produces sufficient to limit the growth of sensitive cells and which is able to withstand the presence of cheaters in its own species. The mixture includes a range of low-rate producers and non-producers, none of which could survive without the presence of the dominant producer strain. We also consider the case of evolution of antibiotic resistance within the sensitive species. In order for the resistant cells to survive, they must grow faster than both the non-producers and the producers. However, if the resistant cells grow too rapidly, the producing species is eliminated, after which the resistance mutation is no longer useful, and sensitive cells take over the system. We show that there is a range of growth rates of the resistant cells where the two species coexist, and where the production mechanism is maintained as a polymorphism in the producing species and the resistance mechanism is maintained as a polymorphism in the sensitive species.Author SummaryNatural environments such as the soil contain many species of antibiotic producing bacteria. Antibiotics prevent the growth of sensitive species that would otherwise outcompete the more-slowly-growing antibiotic producers. The producers are also vulnerable to competition from non-producing “cheats” arising by mutations within the producing species that avoid the metabolic cost of antibiotic production. We consider multiple strains of producers that differ in production rate in the presence of sensitive cells of a different species. We show, in 2d simulations, that the system evolves towards a state with a dominant producer strain that is able to outcompete the sensitive cells, plus a range of low-rate producers and non-producers that can survive in the presence of the dominant producer, but not on their own. This system remains stable, despite the short-term selective advantage to reducing production rate. When resistant mutants are added to the sensitive species, we show that there is a range of growth rate of the resistant cells in which producers, non-producers, sensitive and resistant cells can all coexist - as we see in nature. Our model shows the balance of factors required to maintain resistance mechanisms and production mechanisms together within the mixture of species.