Signature transcriptome analysis of stage specific atherosclerotic plaques of patients

Abstract

AbstractBackgroundInflammation plays an important role in all the stages of atherosclerotic plaque development. The current study aimed at assessing the altered expression of genes functioning in inflammation within the early stage (ES)and advanced stage (AS) atherosclerotic plaques obtained from patients undergoing coronary artery bypass grafting (CABG) surgery and identifying biomarker panel/sthat may detect the status of plaque stages using peripheral blood samples.MethodsA section of ES and ASplaques and normal left internal mammary arteries (LIMA) were obtained from 8 patients undergoing theCABG surgery. Total RNA isolated was analysed for mRNA and miRNAexpression profile by Affymetrix arrays. Significant number of mRNAs was found to be differentially expressed in ES and ASplaque tissues relative to LIMA. The pathway analysis of differentially expressed mRNAs in the two plaque stages was also performed using DAVID Bioinformatics Database.ResultsThe mRNAs were found to be involved in critical inflammatory processes such as Toll-like receptor signalling pathway and cytokine-cytokine receptor interaction. Few miRNAs targeting these mRNAs were also altered in the two plaque conditions. QRT-PCR results showedsimilar expression pattern of few of themRNAs and miRNAsin peripheral blood of same patients relative to healthy controls.ConclusionChanges in mRNA and miRNA expression associated with various inflammatory processes occur in different atherosclerotic stage plaques as well as peripheral blood. Detection of such variations in patients’ blood can be used as a possible prognostic tool to detect and/or predict the risk and stage of atherosclerosis.