Abstract
ABSTRACTPlant growth and fertility strongly depend on environmental conditions such as temperature. Remarkably, temperature also influences meiotic recombination and thus, the current climate change will affect the genetic make-up of plants. To further understand temperature effects on meiosis, we have followed male meiocytes of Arabidopsis thaliana by live cell imaging under three different temperature regimes, at 21°C and at heat shock conditions of 30°C and 34°C as well as after an acclimatization phase of one week at 30°C. This work led to a cytological framework of meiotic progression at elevated temperature. We found that an increase to 30°C, sped up meiotic progression with specific phases being more amenable to heat than others. An acclimatization phase often moderated this effect. A sudden increase to 34°C promoted a faster progression of meiosis in early prophase compared to 21°C. However, the phase in which cross-overs maturate was found to be prolonged at 34°C. Interestingly, mutants involved in the recombination pathway did not show the extension of this phase at 34°C demonstrating that the delay is recombination dependent. Further analysis revealed the involvement of the ATM kinase in this prolongation indicating the existence of a specialized variant of the pachytene checkpoint in plants.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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