Draft genome assembly data of Anoxybacillus sp. strain MB8 isolated from Tattapani hot springs, India

Abstract

AbstractDiscovery of novel thermophiles has shown promising applications in the field of biotechnology. Due to their thermal stability, they can survive the harsh processes in the industries, which make them important to be characterized and studied. Members of Anoxybacillus are alkaline tolerant thermophiles and have been extensively isolated from manure, dairy-processed plants, and geothermal hot springs. This article reports the assembled data of an aerobic bacterium Anoxybacillus sp. strain MB8, isolated from the Tattapani hot springs in Central India, where the 16S rRNA gene shares an identity of 97% (99% coverage) with Anoxybacillus kamchatkensis strain G10. The de novo assembly and annotation performed on the genome of Anoxybacillus sp. strain MB8 comprises of 2,898,780 bp (in 190 contigs) with a GC content of 41.8% and includes 2,976 protein-coding genes,1 rRNA operon, 73 tRNAs, 1 tm-RNA and 10 CRISPR arrays. The predicted protein-coding genes have been classified into 21 eggNOG categories. The KEGG Automated Annotation Server (KAAS) analysis indicated the presence of assimilatory sulfate reduction pathway, nitrate reducing pathway, and genes for glycoside hydrolases (GHs) and glycoside transferase (GTs). GHs and GTs hold widespread applications, in the baking and food industry for bread manufacturing, and in the paper, detergent and cosmetic industry. Hence, Anoxybacillus sp. strain MB8 holds the potential to be screened and characterized for such commercially relevant enzymes.Specifications TableValues of DataThe Anoxybacillus sp. strain MB8 genome assembly data provides insights into functional potential of thermophilic enzymes of this thermotolerant microbe.The presence of genes for Glycoside Hydrolase (GHs) like alpha-amylase, pullulanase, neopullulanase, alpha-glucosidase, beta-fructofuranosidase etc. and genes for Glycosyl Transferase (GTs) like sucrose synthase, maltodextrin phosphorylase, starch synthase, and glycogen phosphorylase were identified, which hold strong industrial values.The taxonomic annotation of Anoxybacillus sp. strain MB8 using different approaches indicates that the closest relatives were Anoxybacillus gonensis NZ CP012152T (96.86% ANI) and Anoxybacillus kamchatkensis G10 NZ CP025535 (96.83% ANI) obtained from. The strain MB8 most likely belongs to the same subspecies of Anoxybacillus gonensis NZ CP012152T.