Abstract
AbstractSpace-based biomanufacturing has the potential to improve the sustainability of deep space exploration. To advance biomanufacturing, bioprocessing systems need to be developed for space applications. Here, commercial technologies were assessed to design space bioprocessing systems to supply a liquid amine carbon dioxide scrubber with active carbonic anhydrase produced recombinantly. Design workflows encompassed biomass dewatering of 1 L Escherichia coli cultures through to recombinant protein purification. Equivalent system mass (ESM) analyses had limited utility for selecting specific technologies. Instead, bioprocessing system designs focused on minimizing complexity and enabling system versatility. Three designs that differed in biomass dewatering and protein purification approaches had nearly equivalent ESM of 357-522 kg eq. Values from the system complexity metric (SCM), technology readiness level (TRL), and degree of crew assistance metric identified a simpler, less costly, and easier to operate design for automated biomass dewatering, cell lysis, and protein affinity purification.
Publisher
Cold Spring Harbor Laboratory
Reference59 articles.
1. Anderson, M. S. et al. NASA Environmental Control and Life Support Technology Development and Maturation for Exploration: 2018 to 2019 Overview. in 49th International Conference on Environmental Systems (2019).
2. Schneider, W. F. et al. NASA Environmental Control and Life Support Technology Development and Maturation for Exploration: 2019 to 2020 Overview. in 50th International Conference on Environmental Systems (2020).
3. Fundamental Understanding of CO2 Capture and Regeneration in Aqueous Amines from First-Principles Studies: Recent Progress and Remaining Challenges;Ind. Eng. Chem. Res,2017
4. CO2 Capture by Tertiary Amine Absorbents: A Performance Comparison Study;Ind. Eng. Chem. Res,2013
5. Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas