Practical guide to calculating impact of avidity in models of bispecific antibodies with two membrane-bound targets

Abstract

AbstractHere we present details of the calculation necessary to estimate the impact of avidity in a mathematical model of a bispecific antibody with two membrane-bound targets. The calculation is used to reproduce the results reported in Rhoden et al. (2016) and implemented in Kareva et al. (2018). We reproduce the impact of difference in relative concentration of the two targets on projections of free and bound concentrations of both targets and the antibody and highlight the applicability of this approach for supporting model informed decision making particularly in the early stages of drug discovery and development.