Abstract
AbstractThe effect of different concentrations of sodium salicylate (NaSA), a phenolic compound, onin vitrocallus and shoot regeneration of cucumber (Cucumis sativusL.) was investigated. Four-day-old cotyledon explants from the Spanish cultivar ‘Marketer’ were employed. MS-derived shoot induction medium containing 0.5 mg L-1 IAA and 2.5 mg L-1 BAP was supplemented with NaSA. Frequency and shoot number were enhanced by 1.5-fold with NaSA at 0.1 μM. Higher salicylate levels led to increased callus formation and decreased shoot regeneration. The application of sodium salicylate at a specific concentration showed a positive trend inin vitroadventitious organogenesis of a commercial cucumber cultivar. Some probable mechanisms that may underlie the beneficial effects of salicylic acid/salicylates onin vitroregeneration were also discussed.
Publisher
Cold Spring Harbor Laboratory