Production of selenium nanoparticles occurs through an interconnected pathway of sulfur metabolism and oxidative stress response inPseudomonas putidaKT2440

Abstract

AbstractThe soil bacteriumPseudomonas putidaKT2440 has been shown to produce selenium nanoparticles aerobically from selenite; however, the molecular actors involved in this process are unknown. Here, through a combination of genetic and analytical techniques, we report the first insights into selenite metabolism in this bacterium. Our results suggest that the reduction of selenite occurs through an interconnected metabolic network involving central metabolic reactions, sulfur metabolism, and the response to oxidative stress. Genes such assucA, D2HGDH and PP_3148 revealed that the 2-ketoglutarate and glutamate metabolism is important to converting selenite into selenium. On the other hand, mutants affecting the activity of sulfite reductase reduced the bacteria’s ability to transform selenite. Other genes related to sulfur metabolism (ssuEF,sfnCE, sqrR,sqrandpdo2) and stress response (gqr,lsfA,ahpCFandsadI) were also identified as involved in selenite transformations. Interestingly, suppression of genessqrR,sqrandpdo2resulted in the production of selenium nanoparticles at a higher rate than the wild-type strain, which is of biotechnological interest. The data provided in this study brings us closer to understanding the metabolism of selenium in bacteria, and offers new targets for the development of biotechnological tools for the production of selenium nanoparticles.