SpaCeNet: Spatial Cellular Networks from omics data

Abstract

Advances in omics technologies have allowed spatially resolved molecular profiling of single cells, providing a window not only into the diversity and distribution of cell types within a tissue, but also into the effects of interactions between cells in shaping the transcriptional landscape. Cells send chemical and mechanical signals which are received by other cells and transduced through signaling cascades from the membrane to the nucleus, where they can subsequently initiate context-specific gene regulatory responses. These interactions and their responses shape the individual molecular phenotype of a cell in a given microenvironment. RNAs or proteins measured in individual cells together with the cells’ spatial distribution provide invaluable information about these mechanisms and the regulation of genes beyond processes occurring independently in each individual cell. “SpaCeNet” is a method designed to elucidate both the intracellular molecular networks (capturing how molecular variables affect each other within the cell) and the intercellular molecular networks (how cells affect molecular variables in their neighbors). This is achieved by estimating conditional independence relations between captured variables within individual cells and by disentangling these from conditional independence relations between variables of different cells.