Genome-wide association study of water use patterns of common bean (Phaseolus vulgaris L.) genotypes in response to drying soil

Author:

Cordoba-Novoa HenryORCID,Kelly James D.,Hoyos-Villegas ValerioORCID

Abstract

AbstractCommon bean (Phaseolus vulgaris L.) is the main legume crop for direct human consumption worldwide, especially in developing countries. Among the abiotic factors affecting common bean production, drought is the most limiting condition. Capacity for the accurate identification and selection of drought-tolerant genotypes is a paramount step in the improvement of new cultivars. This study is aimed at characterizing the genetic variability and genetic architecture of transpiration, stomatal regulation and whole plant water use within the Mesoamerican common bean germplasm. Three greenhouse experiments were conducted, and water use was recorded gravimetrically. Transpiration of water-stressed plants was normalized against well-watered plants to obtain a normalized transpiration rate (NTR). A critical fraction of transpirable soil water (FTSWc) was estimated as the inflection point at which NTR starts decreasing linearly. Genome-wide association (GWA) analyses for average NTR and FTSWc were performed. High variation in average NTR and FTSWc was found among genotypes. Genomic signals (QTL) controlling the variation of these traits were identified on Pv01 and Pv07 some located in intergenic, intronic and exonic regions. A set of novel candidate genes and putative regulatory elements located in these QTL were identified. Most of the genes have been previously reported to be involved in abiotic tolerance, including some of the five transcription factors identified. Candidate genes can be used for marker-assisted selection and further study of drought tolerance mechanisms in common bean. Our results provide tools to accelerate the drought tolerance improvement in the crop.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3