An in-vitro investigation of effects of acetylcholine on attenuate and low motile diluted rooster semen quality

Abstract

AbstractThis study was conducted to evaluate the effects of acetylcholine (ACh) on 24 hours preserved diluted rooster semen at 4°C with a low motility percentage during 2 hours at room temperature incubation. Ten indigenous same-aged roosters were used in this study, adapted by Dorso-abdominal massage for semen collection for one month before starting main work. Semen was collected weekly twice by the Dorso-abdominal massage technique, collected semen transferred to the laboratory in less than 10 minutes, and took a previous qualifying examination. Qualify semen from each rooster polled together and diluted by the Lake extender and preserved for 24 hours at 4°C in the refrigerator. Different ACh concentrations (10mM-100mM) were added to Low motile semen and stored for 24 h at 4°C, and the quality parameters such as motility, viability, acrosome, and plasma membrane integrity were measured evaluated. Adding ACh in 10mM for the low motile semen significantly increased semen motility from 50% to 78.5% (P<0.05) at time zero, but other ACh concentrations did not have any significant differences compared to control. During two hours’ incubation of recovered semen at room temperature, 10mM ACh prevented declining semen motility compared to control and other ACh concentrations significantly (P<0.05), which motility in 10mM ACh concentration 59%. In contrast, control, 1mM ACh, and 100μM ACh group was 2.5%, 4%, and 1%, respectively. Semen viability after two hours of recovery at room temperature significantly 3.5% was less than the control group (P<0.05), but acrosome and plasma membrane integrity has not had any differences between all experimental groups (P>0.05). We can conclude that 10mM ACh can recover semen motility and not have toxicity and side effects on semen quality.