Super-exponential growth and stochastic size dynamics in rod-like bacteria

Abstract

Proliferating bacterial cells exhibit stochastic growth and size dynamics but the regulation of noise in bacterial growth and morphogenesis remains poorly understood. A quantitative understanding of morphogenetic noise control, and how it changes under different growth conditions, would provide better insights into cell-to-cell variability and intergenerational fluctuations in cell physiology. Using multigenerational growth and width data of singleEscherichia coliandCaulobacter crescentuscells, we deduce the equations governing growth and size dynamics of rod-like bacterial cells. Interestingly, we find that bothE. coliandC. crescentuscells deviate from exponential growth within the cell cycle. In particular, the exponential growth rate increases during the cell cycle, irrespective of nutrient or temperature conditions. We propose a mechanistic model that explains the emergence of super-exponential growth from autocatalytic production of ribosomes, coupled to the rate of cell elongation and surface area synthesis. Using this new model and statistical inference on large datasets, we construct the Langevin equations governing cell size and size dynamics ofE. colicells in different growth conditions. The single-cell level model predicts how noise in intragenerational and intergenerational processes regulate variability in cell morphology and generation times, revealing quantitative strategies for cellular resource allocation and morphogenetic noise control in different growth conditions.