Abstract
A novel 15-kDa protein, RbfA, has been identified by virtue of its ability to act as a high copy suppressor of a previously characterized dominant cold-sensitive mutation (C23U) in 16S rRNA. RbfA is found associated with free 30S ribosomal subunits, but not with 70S ribosomes or polysomes, and is essential for maximal cell growth, particularly at low temperatures. Cells lacking RbfA in a wild-type rRNA background exhibit a cold-sensitive phenotype that is strikingly similar to that of the cold-sensitive C23U rRNA mutant. The observed patterns of allele specificity of suppression and synthetic lethality in cells containing an RbfA knockout in combination with various 16S rRNA mutations suggests that RbfA interacts with the 5'-terminal helix region of 16S rRNA, possibly during a late step of 30S maturation.
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Reference49 articles.
1. Mutant DnaK chaperones cause ribosome assembly defects in Escherichia coli.
2. Allen, P. 1992. “Involvement of ribosomal RNA in translational accuracy.” Ph.D. thesis, University of California, Santa Cruz, CA.
3. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
4. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Seidman, and K. Struhl. eds. 1987. Current protocols in molecular biology. Wiley/Greene Publishing, New York.
5. Effects of low temperature on in vivo and in vitro protein synthesis in Escherichia coli and Pseudomonas fluorescens.;J. Bacteriol.,1978
Cited by
142 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献