Immunogenicity assessment of PRRS polylactic acid glycolic acid DNA vaccine

Abstract

AbstractIn order to enhance the immune effect of DNA vaccine, poly(lactide-co-glycolide (PLGA)] microparticles were prepared by solvent evaporation method, and the porcine reproductive and respiratory syndrome (PRRS) DNA vaccine pCI-ORF5 was adsorbed to the porcine reproductive and respiratory syndrome (PRRS) DNA vaccine. The surface of the microparticles was used to detect the amount of DNA adsorbed by PLGA microparticles, in vitro release, and immunogenicity in mice. The results showed that the DNA adsorption capacity of PLGA particles could reach 0.9% within 6h, and the release in vitro was affected by many factors such as CTAB content, PLGA molecular weight, PLGA concentration and internal water phase volume. After immunizing mice with the naked DNA vaccine, PLGA microparticles were found to significantly enhance the humoral and cellular immunity induced by the adsorbed DNA vaccine, indicating that it has a good application prospect as a vector for delivering DNA vaccines.