Author:
Blasius Melanie,Wagner Sebastian A.,Choudhary Chunaram,Bartek Jiri,Jackson Stephen P.
Abstract
RNA metabolism is altered following DNA damage, but the underlying mechanisms are not well understood. Through a 14-3-3 interaction screen for DNA damage-induced protein interactions in human cells, we identified protein complexes connected to RNA biology. These include the nuclear exosome targeting (NEXT) complex that regulates turnover of noncoding RNAs termed promoter upstream transcripts (PROMPTs). We show that the NEXT subunit RBM7 is phosphorylated upon DNA damage by the MAPKAPK2 kinase and establish that this mediates 14-3-3 binding and decreases PROMPT binding. These findings and our observation that cells lacking RBM7 display DNA damage hypersensitivity link PROMPT turnover to the DNA damage response.
Funder
Cancer Research UK
European Community Seventh Framework Programme
CRUK
Wellcome Trust
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Cited by
46 articles.
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