Detection for suspected Genetically Modified Maize and Soybean crops in the selcted places of Ethiopia using PCR machine

Abstract

AbstractDetection of genetically modified organisms (GMOs) in crops is an important issue for all the subjects involved in seed quality control and customer’s right. Due to the increasing number of GMOs research and development activity in the globe during the past few years, it has become necessary to screen and regulate highely practiced GMO crops. In this study, following the extraction of genomic DNA from agriculture farming land and commercial areas collected samples that suspected for GMOs such as Soybean and maize crops, the recombinant DNA target sequences were targeted to detect for transgene such as CaMV35s gene using thermal cycler Polymerase Chain Reaction (PCR). In addition, for positive control taxone specfic Invertase and Lectine gene of maize and soybean have done. Based on the gel electrophoresis results, Invertase and Lectine gene events were detected in all maize and soybean samples respectively. How ever, fortunately in all collected samples of maize and soybean in different places of the countery trans-genes were not detected. Finally, this reserch result concludes that in the studied area soybean and maize crops are GMO free.