Chromatin regulatory dynamics of early human small intestinal development using a directed differentiation model

Abstract

AbstractBackgroundThe establishment of the small intestinal (SI) lineage during human embryogenesis is critical for the proper development of neonatal gut functions, including nutrient absorption and immune defense. The chromatin dynamics and regulatory networks that drive human SI lineage formation and regional patterning are essentially unknown. To fill this knowledge void, we apply a cutting-edge genomic technology to a state-of-the-art human model of early SI development. Specifically, we leverage chromatin run-on sequencing (ChRO-seq) to define the landscape of active promoters, enhancers, super enhancers, and gene bodies across distinct stages of directed differentiation of human pluripotent stem cells (hPSCs) into SI spheroids with regional specification.ResultsThrough comprehensive ChRO-seq analysis we identify candidate stage-specific chromatin activity states, novel markers, and enhancer hotspots during the directed differentiation process. Moreover, we propose a detailed transcriptional network associated with SI lineage formation or initial regional patterning. Among our findings is a unique pattern of enhancer activity and transcription at HOX gene loci that is previously undescribed. Analysis of single cell RNA-seq data from human fetal SI at early developmental time points shed further light on the unique HOX gene temporal dynamics that underlies SI regional patterning.ConclusionsOverall, the results lead to a new proposed working model for the regulatory underpinnings of human SI lineage formation and regional patterning, thereby adding a novel dimension to the literature that has thus far relied almost exclusively on non-human models.