Photoprotective mechanisms inElysiaspecies hostingAcetabulariachloroplasts shed light on host-donor compatibility in photosynthetic sea slugs

Abstract

AbstractSacoglossa sea slugs have garnered attention due to their ability to retain intracellular functional chloroplasts from algae, while degrading other algal cell components. While protective mechanisms that limit oxidative damage under excessive light are well documented in plants and algae, the photoprotective strategies employed by these photosynthetic sea slugs remain unresolved. Species within the genusElysiaare known to retain chloroplasts from various algal sources, but the extent to which the metabolic processes from the donor algae can be sustained by the sea slugs is unclear. By comparing their responses to high light conditions through kinetic analyses, molecular techniques, and biochemical assays, this study highlights significant differences between two photosyntheticElysiaspecies with chloroplasts derived from the green algaAcetabularia acetabulum. Notably,Elysia timidadisplayed remarkable tolerance to high light stress and sophisticated photoprotective mechanisms such as an active xanthophyll cycle, efficient D1 protein recycling, accumulation of heat-shock proteins and α-tocopherol. In contrast,Elysia crispataexhibited absence or limitations in these photoprotective strategies. Our findings emphasize the intricate relationship between the host animal and the stolen chloroplasts, highlighting different capacities to protect the photosynthetic organelle from oxidative damage.