Phospho-Tune: Enhanced Structural Modeling of Phosphorylated Protein Interactions

Abstract

AbstractIn computational biology, accurate prediction of phosphopeptide-protein complex structures is essential for understanding cellular functions and advancing drug discovery and personalized medicine. While AlphaFold has significantly improved protein structure prediction, it faces accuracy challenges in predicting structures of complexes involving phosphopeptides possibly due to structural variations introduced by phosphorylation in the peptide component. Our study addresses this limitation by refining AlphaFold to improve its accuracy in modeling these complex structures. We employed weighted metrics for a comprehensive evaluation across various protein families. The enhanced model notably outperforms the original AlphaFold, showing a substantial increase in the weighted average local distance difference test (lDDT) scores for peptides: from 52.74 to 76.51 in the Top 1 model and from 56.32 to 77.91 in the Top 5 model. These advancements not only deepen our understanding of the role of phosphorylation in cellular signaling but also have extensive implications for biological research and the development of innovative therapies.