Nanoscale interaction of endonuclease APE-1 with DNA characterized by atomic force microscopy

Abstract

AbstractApurinic/apyrimidinic endonuclease 1 (APE1) is involved in DNA replication, repair, and transcriptional regulation mechanisms. This multifunctional activity of APE1 should be supported by specific structural properties of APE1 that have not yet been elucidated. Here we applied atomic force microscopy (AFM) to characterize the interactions of APE1 with DNA. Complexes of APE1 with DNA containing G-rich segments were visualized, and analysis of the complexes revealed the affinity of APE1 to G-rich DNA sequences. Furthermore, loops in the DNA-APE1 complexes were visualized, and their yield was as high as 53 %. However, the loops were non-specific, with quantitative analysis revealing the yield of loops bridging two G-rich DNA segments to be 41%. Analysis of protein size in various complexes was performed, and these data showed that loops are formed by APE1 monomer, suggesting that APE1 has two DNA binding sites. The data lead us to a model for the interaction of APE1 with DNA that describes its molecular site search mechanism. The new properties of APE1 in organizing DNA, by bringing two distant sites together, may be important for facilitating the scanning for damage and coordinating repair and transcription.