Evaluation of a novel Tc-24 recombinant antigen ELISA for serologic testing forTrypanosoma cruziin dogs

Author:

Mejia RojelioORCID,Verocai Guilherme G.,Mosley Ilana A.,Zhan Bin,Vongthavaravat Lindsey,Busselman Rachel E.ORCID,Hamer Sarah A.

Abstract

AbstractChagas disease is a parasitic infection caused byTrypanosoma cruzi. Diagnosis of chroni Chagas disease in dogs relies on limited serological test options. This study used a new Tc-24 recombinant antigen ELISA on an archival set of 70 dog serum samples from multi-dog kennel environments in Texas subjected to three existing Chagas serological tests. Tc-24 ELISA produced a quantitative result and could detect anti-T. cruziantibodies in dogs with high sensitivity and specificity. Comparing individual tests to Tc-24 ELISA resulted in strong associations and correlations, which suggest that Tc-24 ELISA is a reliable and accurate diagnostic tool for dogs with a single test.

Publisher

Cold Spring Harbor Laboratory

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