Chromosome-scale pearl millet genomes reveal aCARLACTONOIC ACID METHYL TRANSFERASEas key determinant of strigolactone pattern and Striga susceptibility

Author:

Kuijer Hendrik NJORCID,Wang Jian YouORCID,Bougouffa Salim,Abrouk MichaelORCID,Jamil Muhammad,Incitti Roberto,Alam IntikhabORCID,Balakrishna Aparna,Alvarez Derry,Votta Cristina,Chen Guan-Ting Erica,Martínez Claudio,Zuccolo Andrea,Berqdar Lamis,Sioud Salim,Fiorilli Valentina,de Lera Angel R,Lanfranco Luisa,Gojobori Takashi,Wing Rod A,Krattinger Simon G,Gao Xin,Al-Babili Salim

Abstract

AbstractThe yield of pearl millet, a resilient cereal crop crucial for African food security, is severely impacted by the root parasitic weedStriga hermonthica,which requires host-released strigolactones (SLs) for seed germination. Herein, we identified four SLs present in the Striga-susceptible line SOSAT-C88-P10 (P10), but absent in the resistant 29Aw (Aw). We generated chromosome-scale genome assemblies including four gapless chromosomes for each line. We found the Striga-resistant Aw lacks a 0.7 Mb genome segment containing two putativeCARLACTONOIC ACID METHYL TRANSFERASE1(CLAMT1) genes. Upon transient expression, P10CLAMT1b produced methyl carlactonoate (MeCLA), an intermediate in SL biosynthesis. Feeding Aw with MeCLA resulted in the production of two P10-specific SLs. Screening a diverse pearl millet panel confirmed the pivotal role of theCLAMT1section for SL diversity and Striga susceptibility. Our results reveal a reason for Striga susceptibility in pearl millet and pave the way for generating resistant lines through marker-assisted breeding or direct genetic modification.

Publisher

Cold Spring Harbor Laboratory

Reference64 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3