Abstract
AbstractEudicot plant species have bifacial leaves with each surface varying in a diversity of components, resulting in potentially different microhabitats for pathogens. We tested howBotrytis cinerea,a necrotroph fungal pathogen, interacts with the two different leaf surfaces across 16 crop species and 20 Arabidopsis genotypes. This showed that the abaxial surface is generally more susceptible to the pathogen than the adaxial surface. In Arabidopsis, the differential lesion area between leaf surfaces was associated to jasmonic acid (JA) and salicylic acid (SA) signaling and differential induction of defense chemistry. When infecting the adaxial surface, leaves mounted stronger defenses by producing more glucosinolates and camalexin defense compounds, partially explaining the differential susceptibility across surfaces. Testing a collection of 96B. cinereastrains showed genetic heterogeneity of growth patterns, with a few strains preferring the adaxial surface while most are more virulent on the abaxial surface. Overall, we show that leaf-Botrytis interactions are complex with host-specific, surface-specific, and strain-specific behaviors. Within Arabidopsis, this mechanistically links to potential variation in JA/SA signaling across the two surfaces.
Publisher
Cold Spring Harbor Laboratory
Reference65 articles.
1. Arabidopsis myrosinases TGG1 and TGG2 have redundant function in glucosinolate breakdown and insect defense
2. Killing softly: A roadmap of Botrytis cinerea pathogenicity;Trends in plant science,2022
3. Killing softly: a roadmap of Botrytis cinerea pathogenicity;Trends in plant science,2023
4. Bu Q , Jiang H , Li C-B , Zhai Q , Zhang J , Wu X , Sun J , Xie Q , Li C 2008. Role of the Arabidopsis thaliana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses. Cell Res. 756–767.
5. How does a plant orchestrate defense in time and space? Using glucosinolates in Arabidopsis as case study;Current opinion in plant biology,2017