A screen of mutants generated and imaged by the International Mouse Phenotyping Consortium identifies 39 novel genes regulating the development of the secondary palate

Abstract

AbstractThe International Mouse Phenotyping Consortium (IMPC) has generated thousands of knockout mouse lines, of which a large proportion is embryonic or early neonatal lethal. The IMPC has generated and imaged embryos from lethal lines and made the three-dimensional image data sets publicly available. In this study, we used this resource to screen homozygous null mutants for defects in the development of the secondary palate. Altogether, we analyzed optical sections from 3216 embryos isolated at embryonic day (E) 15.5 and E18.5 from 478 homozygous mutant lines. Through this analysis, we discovered 39 novel genes important for palatal development. These studies provide new insights into the molecular regulation of palatogenesis and craniofacial disease and offer a useful resource for future exploration.