Linear contraction of stress fibers generates cell body rotation

Abstract

AbstractWounds are healed by crawling migration of the epidermal cells around the injured area. Fish epidermal keratocytes that rapidly repair wounds comprise a frontal crescent-shaped lamellipodium and a rear rugby ball-shaped cell body. The cell body rotates like a wheel during migration. Stress fibers, which are bundles of contractile actomyosin filaments, are arranged along the seams of the rugby ball. Here we show the linear contraction of stress fibers to be the driving force for rotation. We constructed a mechanical model of the cell body that consisted of a soft cylinder with a contractile coil. From the motion of the model, it was predicted that contraction of the stress fibers would deform the soft cell body, as a result of which the deformed cell body would push against the substrate to generate torque. This prediction was confirmed by the observation of stress fiber dynamics in migrating cells. Linear-to-rotation conversion in migrating keratocytes is realized by simple soft-body mechanics. Conversion from linear motion to rotation is widely used in machines with moving parts, but requires somewhat complicated mechanics. An understanding of linear-to-rotation conversion in keratocytes has potential for use in the design of biomimetic soft robots.