At-home saliva sampling in healthy adults using CandyCollect, a lollipop-inspired device

Author:

Tu Wan-chenORCID,McManamen Anika M.ORCID,Su XiaojingORCID,Jeacopello Ingrid,Takezawa Meg G.ORCID,Hieber Damielle L.,Hassan Grant W.,Lee Ulri N.ORCID,Anana Eden V.,Locknane Mason P.,Stephenson Molly W.ORCID,Shinkawa Victoria A. M.,Wald Ellen R.ORCID,DeMuri Gregory P.ORCID,Adams KarenORCID,Berthier ErwinORCID,Thongpang SanittaORCID,Theberge Ashleigh B.ORCID

Abstract

ABSTRACTRespiratory infections are common in children, and there is a need for user-friendly collection methods. Here, we performed the first human subjects study using the CandyCollect device, a lollipop inspired saliva collection device.1We showed the CandyCollect device can be used to collect salivary bacteria from healthy adults usingStreptococcus mutansandStaphylococcus aureusas proof-of-concept commensal bacteria. We enrolled healthy adults in a nationwide (USA) remote study in which participants were sent study packages containing CandyCollect devices and traditional commercially available oral swabs and spit tubes. Participants sampled themselves at home, completed usability and user preference surveys, and mailed the samples back to our laboratory for analysis by qPCR. Our results showed that for participants in which a given bacterium (S. mutansorS. aureus) was detected in one or both of the commercially available methods (oral swab and/or spit tubes), CandyCollect devices had a 100% concordance with the positive result (n=14 participants). Furthermore, the CandyCollect device was ranked the highest preference sampling method among the three sampling methods by 26 participants surveyed (combining survey results across two enrollment groups). We also showed that the CandyCollect device has a shelf life of up to 1 year at room temperature, a storage period that is convenient for clinics or patients to keep the CandyCollect device and use it any time. Taken together, we have demonstrated that the CandyCollect is a user-friendly saliva collection tool that has the potential to be incorporated into diagnostic assays in clinic visits and telemedicine.For Table of Contents Only

Publisher

Cold Spring Harbor Laboratory

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