Phylogenetics and phylogeography ofEuphorbia canariensisreveal an extreme Canarian-Asian disjunction and limited inter-island colonization

Abstract

AbstractEuphorbia canariensisis an iconic endemic species of the Canary Islands and one of the most characteristic species of lowland xerophytic communities known, in Spanish, as ‘cardonal-tabaibal’. This species is widely distributed in the archipelago, which contrasts with the theoretically low dispersal abilities suggested by its unspecialized diasporas. Furthermore, the phylogenetic relationships of this species are unclear, although it is thought to be related to the IndianE. epiphylloidesand not to other cactus-likeEuphorbiaof the Canary Islands (E. handiensis) and Africa. Here we aimed to reconstruct the evolutionary history ofE. canariensisat two levels: (i) a phylogenetic approach aimed at unravelling relationships of this species and large-scale biogeographic patterns, and (ii) a phylogeographic approach focused on the history of colonization between islands of the Canarian archipelago in relation to habitat availability for this species through time. Based on previous phylogenetic studies ofEuphorbia, we sequenced the ITS region forE. canariensisand several potentially related species to build a phylogenetic framework. We also sequenced two cpDNA regions for 92 individuals from 29 populations ofE. canariensisrepresenting its distribution range. We estimated the number of inter-island colonization events using PAICE, a recently developed method that includes a sampling effort correction. Additionally, we used species distribution modelling (SDM) to project current habitat availability forE. canariensisto past periods. Phylogenetic results supported the CanarianE. canariensisas closely related to the Southeast AsianE. epiphylloidesandE. sessiliflora. In the Canarian archipelago,E. canariensisdisplayed a surprising west-to-east colonization pattern. The estimated number of inter-island colonization events was c. 20 – 50, and SDM suggested an increase in habitat availability in recent times. In summary, in this study we confirmed an extreme biogeographic disjunction between Macaronesia and Southeast Asia, described only for a small number of plant species, and estimated relatively low dispersal capabilities forE. canariensis, with a west-to-east colonization pattern in the Canary Islands.HighlightsThe CanarianEuphorbia canariensisis sister to Southeast Asian species.This lineage shows one of the widest disjunctions affecting Macaronesian plants.The lack of intermediate relatives could be the result of extinction events.E. canariensisdisplays low colonization ability in the Canarian archipelago.