Abstract
AbstractThe skin microenvironment created by keratinocytes (KC) influences stress responses of melanocytes (MC) to UVB insult. Here, we investigated paracrine factors involved in the regulatory role of microenvironment created by KC in UVB-mediated MC responses using RNA sequencing analysis as well asin vitroandin vivomodels. RNA-Seq showed that G-CSF and CCL20 genes were highly upregulated in UVB-irradiated KC and their levels best correlated with paracrine protective effects of KC on stress responses of MC to UVB. Recombinant G-CSF and CCL20 treatment revealed the strongest modulatory effects on UVB-induced MC responses by mitigating apoptosis and ROS formation and upregulating tyrosinase and tyrosinase-related protein-1 (TRP-1) involved in the melanogenic pathway. A similar correlation between G-CSF and CCL20 expression in KC and the tyrosinase level in MC was also observed in the UVB-irradiated mouse skin. Our study reports for the first time that G-CSF and CCL20 might play a regulatory role in the KC’s paracrine effects on UVB-mediated MC damage and also provides translational insights for the development of biomarkers for predicting susceptibility to photodamage.Graphical abstract
Publisher
Cold Spring Harbor Laboratory