Cold tolerance and diapause within and across trophic levels: endoparasitic wasps and their fly host have similar phenotypes

Abstract

AbstractLow temperatures associated with winter can limit the survival of organisms, especially ectotherms whose body temperature is similar to their environment. Important adaptations for overwintering such as cold hardiness and diapause have been well-explored in many insect taxa. However, there is a gap in understanding how overwintering may vary among groups of species that interact closely, such as multiple parasitoid species that attack the same host insect. Our study investigated cold tolerance and diapause phenotypes in three endoparasitoid wasps of the apple maggot flyRhagoletis pomonella(Diptera: Tephritidae):Utetes canaliculatus, Diachasma alloeum, andDiachasmimorpha mellea(Hymenoptera: Braconidae). Using a combination of respirometry and eclosion tracking, we detected diapause phenotypes in all three wasp species, remarkably similar to the fly host. Weak diapause was rare (< 5%) in all three wasp species, and while mostD. mellea(93%) entered prolonged diapause under warm conditions, the majority ofU. canaliculatus(92%) andD. alloeum(72%) averted diapause (non-diapause). There was limited interspecific variation in acute cold tolerance among the three wasp species: wasps and flies had similarly high survival (>87%) following exposure to extreme low temperatures (- 20°C) as long as their body fluids did not freeze. The wasp species showed little interspecific variation in survival following prolonged exposure to mild chilling of 8 or more weeks at 4°C. This study shows remarkable conservation of cold tolerance and diapause phenotypes within and across trophic levels. The interaction between diapause phenotype and cold hardiness in these parasitoids is an interesting direction for future research.Graphical Abstract and HighlightsThe apple maggot fly and its parasitoids exhibit the same three diapause phenotypesEach parasitoid wasp species exhibits different proportions of these phenotypesUtetes canaliculatusandDiachasma alloeumare freeze-avoidant, like their host flyThese wasps and flies survive to similarly extreme low temperatures (c. -20°C)Each wasp species survives prolonged exposure to mild chilling (4°C) similarly well