Rare disease research workflow using multilayer networks elucidates the molecular determinants of severity in Congenital Myasthenic Syndromes
Author:
Núñez-Carpintero IkerORCID, O’Connor Emily, Rigau MariaORCID, Bosio MattiaORCID, Spendiff SallyORCID, Azuma YoshiteruORCID, Topf Ana, Thompson RachelORCID, ’t Hoen Peter A.C.ORCID, Chamova Teodora, Tournev Ivailo, Guergueltcheva Velina, Laurie Steven, Beltran SergiORCID, Capella SalvadorORCID, Cirillo DavideORCID, Lochmüller HannsORCID, Valencia AlfonsoORCID
Abstract
AbstractExploring the molecular basis of disease severity in rare disease scenarios is a challenging task provided the limitations on data availability. Causative genes have been described for Congenital Myasthenic Syndromes (CMS), a group of diverse minority neuromuscular junction (NMJ) disorders; yet a molecular explanation for the phenotypic severity differences remains unclear. Here, we present a workflow to explore the functional relationships between CMS causal genes and altered genes from each patient, based on multilayer network analysis of protein-protein interactions, pathways and metabolomics.Our results show that CMS severity can be ascribed to the personalized impairment of extracellular matrix components and postsynaptic modulators of acetylcholine receptor (AChR) clustering. We explore this in more detail for one of the proteins not previously associated with the NMJ, USH2A. Loss of the zebrafish USH2A ortholog revealed some effects on early movement and gross NMJ morphology.This work showcases how coupling multilayer network analysis with personalized -omics information provides molecular explanations to the varying severity of rare diseases; paving the way for sorting out similar cases in other rare diseases.
Publisher
Cold Spring Harbor Laboratory
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