Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19

Author:

Okude Miho,Suzuki Kenji,Naito Asami,Ebashi Akio,Kusama Tomoka,Kiyotaki Junichi,Akashi Yusaku,Kiyasu Yoshihiko,Kurihara Yoko,Notake Shigeyuki,Takanashi Masaki,Setoyama Tomokazu,Kawakami Yasushi,Suzuki Hiromichi

Abstract

AbstractIntroductionWe newly designed and developed two types of hydrogen fuel cell (HFC) buses (motorcoach type and minibus type) with a mobile laboratory system. Feasibility studies have been performed for mobile laboratory testing, especially for the laboratory performance of COVID-19 RT-PCR (PCR).MethodsWe evaluated the driving range capability, PCR sample size capacity, turn-around time (TAT), and analytical performance for the detection of SARS-CoV-2. Saliva samples were used for the current research and the analytical performance was compared with reference PCR.ResultsThe estimated driving range and sample size capacity were 432 km and 3,258 samples, respectively for the HFC motorcoach and 313 km and 2,146 samples for the HFC minibus, respectively. For the TAT, the median time between the sample submission and the completion of PCR were 86 min for the motorcoach and 76 min for the minibus, and the median time between sample submission and the electronic reporting of the result to each visitor were 182 min for the motorcoach and 194 min for the minibus. A secondary analysis of 1,574 HFC mobile laboratory testing samples was conducted and all negative samples were negative by reference PCR. Furthermore, all positive samples were confirmed as positive by reference PCR or other molecular examinations.ConclusionWe confirmed the feasibility of HFC mobile laboratory systems for achieving the rapid reporting of highly accurate PCR results.

Publisher

Cold Spring Harbor Laboratory

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