Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants-Reference-Cited by-同舟云学术

Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants

Published:2023-02-02 Issue: Volume: Page:
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Author:

Gonzalez Veronica,Li Lin,Buarpung Sirirak,Prahl Mary,Robinson Joshua F.,Gaw Stephanie L.

Abstract

SummaryDespite universal recommendations for COVID-19 mRNA vaccination in pregnancy, uptake has been lower than desired. There have been limited studies of the direct impact of COVID-19 mRNA vaccine exposure in human placental tissue. Using a primary human villous explant model, we investigated the uptake of two common mRNA vaccines (BNT162b2 Pfizer-BioNTech or mRNA-1273 Moderna), and whether exposure altered villous cytokine responses. Explants derived from second or third trimester chorionic villi were incubated with vaccines at supraphysiologic concentrations and analyzed at two time points. We observed minimal uptake of mRNA vaccines in placental explants by in situ hybridization and quantitative RT-PCR. No specific or global cytokine response was elicited by either of the mRNA vaccines in multiplexed immunoassays. Our results suggest that the human placenta does not readily absorb the COVID-19 mRNA vaccines nor generate a significant inflammatory response after exposure.

Publisher

Cold Spring Harbor Laboratory

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