Abstract
AbstractProgrammed cell death occurring during plant development (dPCD) is a fundamental process integral for plant growth and reproduction. Here, we investigate the connection between developmentally controlled PCD and fungal accommodation inArabidopsis thalianaroots, focusing on the root cap-specific transcription factor ANAC033/SOMBRERO (SMB) and the senescence-associated nuclease BFN1. Mutations of both dPCD regulators increase colonization by the beneficial fungusSerendipita indica, primarily in the differentiation zone.smb-3mutants additionally exhibit hypercolonization around the meristematic zone and a delay ofS. indica-induced root-growth promotion. This demonstrates that root cap dPCD and rapid post-mortem clearance of cellular corpses represent a physical defense mechanism restricting microbial invasion of the root. Additionally, reporter lines and transcriptional analysis revealed thatBFN1expression is downregulated duringS. indicacolonization in mature root epidermal cells, suggesting transcriptional control as a means for microbial regulation of host dPCD.Key findings in bullet pointsThe process of programmed cell death in root development (dPCD) influences the extent and outcomes of fungal symbiosisFungal colonization of the root tip and differentiation zone is restricted by SMB-mediated clearance of dead cells, which preserves the meristem and regulates symbiosisThe plant nuclease BFN1, which is associated with senescence, is exploited by beneficial microbes to facilitate their root accommodation
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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