Abstract
AbstractCellulose is an economically important source of food, paper, textiles, and biofuel. As an essential component of plant cell walls, cellulose is critical for plant cell growth. Despite its economic and biological significance, the regulation of cellulose biosynthesis is poorly understood. Phosphorylation and dephosphorylation of cellulose synthases (CESAs) were shown to impact the direction and velocity of cellulose synthase complexes (CSCs). Despite a high prevalence of phosphorylation sites in CESAs, the protein kinases that phosphorylate CESAs are largely unknown. Here, we demonstrate that CALCIUM-DEPENDENT PROTEIN KINASE32 (CPK32) regulates cellulose biosynthesisviaphosphorylation of CESA3. Phosphorylation of CESA3 is important for the motility and stability of CSCs. Hence, we uncovered a new function of CPKs that regulates cellulose biosynthesis and a novel mechanism by which phosphorylation regulates the stability of CSCs.
Publisher
Cold Spring Harbor Laboratory
Reference58 articles.
1. Phosphorylation of basic amino acid residues in proteins: important but easily missed;Acta biochimica Polonica,2011
2. F. Ardito , M. Giuliani , D. Perrone , G. Troiano , L. L. Muzio (2017) The crucial role of protein phosphorylation in cell signalingand its use as targeted therapy (Review). in International Journal of Molecular Medicine (Spandidos Publications), pp 271–280.
3. Mass-spectrometry-based draft of the Arabidopsis proteome;Nature,2020
4. H. Wang , D. Chevalier , C. Larue , S. K. Cho , J. C. Walker , The Protein Phosphatases and Protein Kinases of Arabidopsis thaliana. The Arabidopsis Book / American Society of Plant Biologists 5 (2007).
5. CDK5 phosphorylates DRP1 and drives mitochondrial defects in NMDA-induced neuronal death