Acute stress modulates social approach and social maintenance in adult zebrafish

Abstract

ABSTRACTStress alters social functioning in a complex manner. An important variable determining the final effects of stress is stressor intensity. However, the precise relationship between stressor intensity and social behavior is not well understood. Here we investigate the effects of varying acute stressor intensity exposure on social behavior using adult zebrafish. We first establish a novel test using adult zebrafish that allows distinguishing fish’s drive to approach a social cue and its ability to engage and maintain social interaction within the same behavioral paradigm. Next, we combined this test with a new method to deliver an acute stress stimulus of varying intensities. Our results show that both social approach and social maintenance are reduced in adult zebrafish upon acute stress exposure in an intensity-dependent manner. Interestingly, lower stress intensity reduces social maintenance without affecting the social approach, while a higher stress level is required to alter social approach. These results provide evidence for a direct correlation between acute stressor intensity and social functioning and suggest that distinct steps in social behavior are modulated differentially by the acute stress level.SIGNIFICANCE STATEMENTAcute stress exposure has a potent effect on social behavior in many animals including humans. However, so far the effect of different stressor intensity on distinct steps of social behavior has not been directly tested. Here, using zebrafish, we develop a new social behavior paradigm and a new graded acute stressor delivery method in order to test the relationship between acute stressor intensity and social behavior. Our results show that acute stress modulates both social approach and social maintenance in an intensity-dependent manner but social maintenance is affected at lower stress intensity than social approach. Thus, this work reveals that distinct steps in social behavior are differentially modulated by the acute stress level.