A Protein Needle Facilitates Encapsulation of Target Proteins <i>via</i> <i>In-Cell</i> Protein Crystallization-Reference-Cited by-同舟云学术

A Protein Needle Facilitates Encapsulation of Target Proteins via In-Cell Protein Crystallization

Published:2023-08-03 Issue:9 Volume:52 Page:720-723
ISSN:0366-7022
Container-title:Chemistry Letters
language:en
Short-container-title:

Author:

Pham Thuc Toan¹,Abe Satoshi¹,Hirata Kunio²,Katayama Kazuhiko³,Ueno Takafumi¹⁴

Affiliation:

1. School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501 , Japan

2. SR Life Science Instrumentation Unit, RIKEN/SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 , Japan

3. Laboratory of Viral Infection Control, Graduate School of Infection Control Science, Ōmura Satoshi Memorial Institute, Kitasato University, Minato-ku, Tokyo 108-8641 , Japan

4. Living Systems Materialogy (LiSM) Research Group, International Research Frontiers Initiative (IRFI), Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501 , Japan

Abstract

Abstract We have established a new method for encapsulating superfold green fluorescent protein (sfGFP) into polyhedra crystal (PhC) with high efficiency using a protein needle (PN). H1-sfGFP-PN, in which PN is fused to the C-terminus of H1-sfGFP, exhibits a 55-fold increase in encapsulation of sfGFP relative to H1-sfGFP with the improvement provided by the synergistic effects of the H1-helix, PN, and the His-tag at the C-terminus of PN. This method is expected to provide useful materials based on encapsulation of various functional proteins.

Publisher

Oxford University Press (OUP)

Subject

General Chemistry

Link

https://academic.oup.com/chemlett/article-pdf/52/9/720/56205328/cl.230282.pdf

Reference33 articles.

1. Engineering of protein crystals for use as solid biomaterials

2. Porous crystals as scaffolds for structural biology

3. Dynamic, Polymer-Integrated Crystals for Efficient, Reversible Protein Encapsulation

4. Hyperexpandable, self-healing macromolecular crystals with integrated polymer networks