Evaluating Wheat Microsatellite Markers for the Use in Genetic Analysis ofThinopyrum, Dasypyrum,andPseudoroegneriaSpecies

Abstract

A set of 42 SSRs of wheat were evaluated for their cross-amplification on the DNA ofThinopyrum ponticum, Thinopyrum intermedium, Thinopyrum elongatum, Thinopyrum bessarabicum, Pseudoroegneria stipifolia,andDasypyrum villosum.The number of the wheat SSR markers that amplified DNA fragments with determined size forTh. ponticumwas 33 (78.6%); forTh. intermedium,28 (66.7%); forTh. elongatum,24 (57.1%); forTh. bessarabicum,24 (57.1%); forP. stipifolia,26 (69.1%); and forD. villosum,29 (69.0%). Twenty-four primer pairs of wheat SSR markers were successfully amplified from all investigated species. The dataset can be used for phylogenetic studies of wild relatives of wheat, for the estimation of their diversity, and for the introgression of agronomically valuable genes into wheat genome.