Application of Isoelectric Focusing in Thin-Layer Polyacrylamide Gels to the Study of Opine Dehydrogenases in Marine Invertebrates

Author:

Kanno Nobuhiro1,Kamimura Kunihide1,Nagahisa Eizou1,Sato Minoru2,Sato Yoshikazu1

Affiliation:

1. School of Fisheries Sciences, Kitasato University

2. Faculty of Agriculture, Tohoku University

Publisher

Japanese Society of Fisheries Science

Subject

Aquatic Science

Reference20 articles.

1. 1) K. Urich: Comparative Animal Biochemistry (Translated from German by P. J. King), Springer-Verlag, Berlin, 1994, pp. 514-561.

2. 2) G. Gäde and M. K. Grieshaber: Pyruvate reductases catalyze the formation of lactate and opines in anaerobic invertebrates. Comp. Biochem. Physiol., 83 B, 255-272 (1986).

3. 3) K. B. Storey and J. M. Storey: Kinetic characterization of tissuespecific isozymes of octopine dehydrogenase from mantle muscle and brain of Sepia officinalis: functional similarities to the M4 and H4 isozymes of lactate dehydrogenase. Eur. J. Biochem., 93, 545-552 (1979).

4. 4) C. S. Hammen and R. C. Bullock: Opine oxidoreductases in brachi-opods, bryozoans, phoronids and molluscs. Biochem. Syst. Ecol.,19, 263-269 (1991).

5. 5) C. S. Hammen and C. Fielding: Opine oxidoreductases in marine worms of five phyla. Comp. Biochem. Physiol., 106B, 989-992(1993).

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