Tissue Preservation and Total DNA Extraction form Fish Stored at Ambient Temperature Using Buffers Containing High Concentration of Urea

Author:

Asahida Takashi1,Kobayashi Takanori2,Saitoh Kenji3,Nakayama Ichiro4

Affiliation:

1. Tohoku National Fisheries Research Institute

2. National Research Institute of Aquaculture

3. Tohoku National Fisheries Research Institute Hachinohe Branch

4. National Research Institute of Aquaculture Tamaki Branch

Publisher

Japanese Society of Fisheries Science

Subject

Aquatic Science

Reference7 articles.

1. 1) D. S. Proebstel, R. P. Evans, D. K. Shiozawa, and R. N. Williams: Preservation of nonfrozen tissue samples from a salmonine fish Brachymystax lenok (Pallas) for DNA analysis. J. lchthyol. 32, 9-17 (1993).

2. 2) P. S. White and L. D. Densmore III: Mitochondrial DNA isolation, in “Molecular genetic analysis of populations” (ed. by A. R. Hoelzel), Oxford univ. press, Oxford, 1992, pp. 29-58.

3. 3) L. Vigilant, R. Pennington, H. Harpending, T. D. Kocher, and A. C. Wilson: Mitochondrial DNA sequences in single hairs from a southern African population. Proc. Natl. Acad. Sci. USA, 86, 9350-9354 (1989).

4. 4) T. D. Kocher, W. K. Thomas, A. Meyer, S. Y. Edwards, S. Paabo, F. X. Vilablanca, and A. C. Wilson: Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. USA, 86, 6196-6200 (1989).

5. 5) K. Numachi, T. Kobayashi, K. H. Chang, and Y. S. Lin.: Genetic identification and differentiation of the formosan landlocked salmon, Oncorhynchus masou formosanus by restriction analysis of mitochondrial DNA. Bull. Inst. Zool. Acad. Sin., 29, 61-72 (1990).

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