DNA Reduction of Waterborne E.coli by Underwater Capillary Discharge

Abstract

Abstract: Escherichia coli (E. coli), (190CRC) is one of the most common waterborne pathogens. It exists in drinking water and most of the water diseases are associated with it. The permanent inactivation of E.coli from water requires its DNA distortion, that makes it ineffective for regeneration. The underwater plasma discharge in capillary tube induces reactive oxidant species {OH^. radicals, Ozone (O3), hydrogen peroxide (H2O2) and reactive oxygen (O)}. Ultraviolet (UV) radiations are most important shock waves that play a vital role for complete degradation of E.coli from water. In the current study, the effects of plasma for different oxygen injection rates and applied voltages on DNA distortion of E.coli have been reported. The bactericidal tests including DNA and protein leakage of plasma-treated water showed complete distortion of E.coli DNA structure and sterilization of water from bacteria. Keywords: Capillary discharge, E.coli, DNA, Protein leakage, Sterilization.